Nondumiso Ayanda Mazibukoa, Kuo‑Liang Yanga,b*
aDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan; bLaboratory of Immunogenetics, Tzu Chi Cord Blood Bank, and Buddhist Tzu Chi Bone Marrow Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan
Open Access funded by Buddhist Compassion Relief Tzu Chi Foundation
Abstract
Objectives: Here, we show two probable haplotypes associated with the human leukocyte antigen (HLA) DRB1*13:50:01 allele. The haplotypes were observed from 41 randomized unrelated Taiwanese individuals among a population of 23,064 individuals tested. Materials and Methods: The samples in this study were blood samples, preserved in dipotassium ethylenediaminetetraacetic acid and/or ACD anticoagulants. The population is of donors from Tzu Chi Bone Marrow Donor Registry. Allele typing was performed using the sequence‑based typing method, Sanger’s sequencing. To discern the HLA‑A and HLA‑B alleles, exons 2 and 3 were sequenced. For DRB1 alleles, exon 2 was sequenced. Target exon sequence amplifications were done by a polymerase chain reaction and the resulting amplicons were sequenced by Bigdye Terminator Cycle Sequencing Ready Reaction kit, according to the manufacturer’s protocols. Results: Two probable haplotypes that are associated with the DRB1*13:50:01 were observed among the 23,064 Taiwanese randomized unrelated individuals. One of the haplotypes is observed in 39 individuals while the other in two individuals. Conclusion: The findings in this study may be useful in studies reinforcing the understanding and clinical application of the polymorphism of HLA genes and haplotypes.
Keywords: DRB*13:50:01, Haplotypes, Human leukocyte antigen, Taiwanese
