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Development of an enhanced sensitivity method for lateral flow immunoassays: An overview of strategies

Chung‑An Chena,b, Chi‑Hung Liua, Natalie Yi‑Ju Hoa, Sheng Liua, Tsung‑Ting Tsaia,b*

aDepartment of Orthopaedic Surgery, Spine Section and Bone and Joint Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan, bSchool of Medicine, Chang Gung University, Taoyuan, Taiwan

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Open Access funded by Buddhist Compassion Relief Tzu Chi Foundation

 

Abstract
 
Lateral flow immunoassay (LFIA) is a widely recognized point‑of‑care diagnostic platform for detecting various analytes. It must meet the criteria of REASSURED, but poor sensitivity and lack of precision in test results remain the main bottlenecks of LFIA. Therefore, signal enhancement methods, such as devices, nanoprobes, or detection modes, can improve this platform. In this paper, many representative studies from the past decade on LFIA sensing platforms will be discussed in detail.

 

Keywords: Lateral flow immunoassay, Point‑of‑care, Sensing system

 

 

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