Hsuan‑Li Huanga,b, Lu‑Kai Wangc, Fu‑Ming Tsaid*
aDivision of Cardiology, Department of Internal Medicine, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei, Taiwan, bSchool of Post‑Baccalaureate Chinese Medicine, Tzu Chi University, Hualien, Taiwan, cOperation & Promotion Division, National Laboratory Animal Center, National Institutes of Applied Research, Taipei, Taiwan, dDepartment of Research, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei, Taiwan
Open Access funded by Buddhist Compassion Relief Tzu Chi Foundation
Absract
Objectives: The objective is to evaluate the protective effects of six coumarin derivatives against peroxide‑induced cardiomyocyte damage and investigate their action mechanisms. Materials and Methods: Intracellular reactive oxygen species and mitochondrial membrane potential (MMP) were analyzed using dihydrorhodamine 123 and JC‑1 combined with flow cytometry. Cell viability and apoptosis were assessed using WST‑1 and lactate dehydrogenase analysis kits, respectively. The apoptotic signaling pathway was analyzed using a mouse apoptosis array kit. Cellular protein expression was detected using Western blotting. Results: Among the six coumarin derivatives tested, only 7‑hydroxyflavone demonstrated the ability to protect cardiomyocytes from hydrogen peroxide (H2O2)‑induced damage. Protein expression analysis revealed that 7‑hydroxyflavone reduced cytochrome c release from the mitochondria and inhibited H2O2‑induced activation of caspase‑3. In addition, 7‑hydroxyflavone maintained MMP stability in cardiomyocytes exposed to H2O2. Conclusion: 7‑hydroxyflavone has potential as an effective antioxidant supplement for cardiac tissues. Further research is required to elucidate its pharmacokinetics and metabolic profile in humans to facilitate its therapeutic application.
Keywords: 7‑hydroxyflavone, Cardiomyocytes, Coumarin derivatives, Mitochondrial membrane potential, Reactive oxygen species
