Hsuan-Shun Huanga, Sung-Chao Chub, Tang-Yuan Chua, c, d
a Cervical Cancer Prevention Center, Department of Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
b Department of Hematology and Oncology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
c Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
d Institute of Medical Science, Tzu Chi University, Hualien, Taiwan
Abstract
Objective
Reactive oxygen species (ROS)-induced DNA double-strand breaks (DSBs) are a feature of cancer initiation. Recently, the cells of origin of ovarian high-grade serous carcinoma (HGSC) have been identified as secretory cells in fallopian tube fimbriae, which might be heavily exposed to ROS after ovulation. Establishing a sensitive detection method for measuring DSBs and the cell cycle in fallopian tube secretory cells after ovulation is essential for facilitating research on ovarian cancer formation.
Materials and methods
Fimbrial epithelial cells from a prophylactically removed human fallopian tube were primarily cultured and immortalized by TP53/Rb-null and hTERT overexpression (FE25 cell line). Hydrogen peroxide (H2O2) was used to treat the FE25 cells to induce DNA DSBs. The DSBs and cell cycle were analyzed using flow cytometry. In addition, human specimens of fimbrial scrapings were subjected to the flow cytometry-based analysis.
Results
We report an efficient flow cytometry-based method for analyzing the DSBs and cell cycle in immortalized fallopian tube secretory cells as well as in clinical specimens.
Conclusion
This analysis method would facilitate the investigation of cancer initiation in fallopian tube fimbriae and other tissues with DSBs.
Keywords
DNA double-strand break (DSB); Fallopian tube fimbriae; Flow cytometry; Reactive oxygen species (ROS)